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Fig. 3 Effect of OSM on JAK2/STAT3 signalling pathway during early and middle osteogenic differentiation of tendon stem cells. (A) After OSM-induced osteogenic differentiation of tendon stem cells for 7 days, expression of <t>OSMR,</t> <t>GP130,</t> p-JAK2, and p-STAT3 proteins was detected by western blotting (n = 3). Two-tailed Student’s t-test. (B)Fourteen days after OSM-induced osteogenic differentiation of tendon stem cells, expression of OSMR, GP130, p-JAK2, and p-STAT3 proteins was detected by western blotting (n = 3). Two-tailed Student’s t-test.Data are presented as means ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001
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Fig. 3 Effect of OSM on JAK2/STAT3 signalling pathway during early and middle osteogenic differentiation of tendon stem cells. (A) After OSM-induced osteogenic differentiation of tendon stem cells for 7 days, expression of <t>OSMR,</t> <t>GP130,</t> p-JAK2, and p-STAT3 proteins was detected by western blotting (n = 3). Two-tailed Student’s t-test. (B)Fourteen days after OSM-induced osteogenic differentiation of tendon stem cells, expression of OSMR, GP130, p-JAK2, and p-STAT3 proteins was detected by western blotting (n = 3). Two-tailed Student’s t-test.Data are presented as means ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001
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Fig. 3 Effect of OSM on JAK2/STAT3 signalling pathway during early and middle osteogenic differentiation of tendon stem cells. (A) After OSM-induced osteogenic differentiation of tendon stem cells for 7 days, expression of OSMR, GP130, p-JAK2, and p-STAT3 proteins was detected by western blotting (n = 3). Two-tailed Student’s t-test. (B)Fourteen days after OSM-induced osteogenic differentiation of tendon stem cells, expression of OSMR, GP130, p-JAK2, and p-STAT3 proteins was detected by western blotting (n = 3). Two-tailed Student’s t-test.Data are presented as means ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001

Journal: Journal of orthopaedic surgery and research

Article Title: Oncostatin M promotes osteogenic differentiation of tendon-derived stem cells through the JAK2/STAT3 signalling pathway.

doi: 10.1186/s13018-024-04915-5

Figure Lengend Snippet: Fig. 3 Effect of OSM on JAK2/STAT3 signalling pathway during early and middle osteogenic differentiation of tendon stem cells. (A) After OSM-induced osteogenic differentiation of tendon stem cells for 7 days, expression of OSMR, GP130, p-JAK2, and p-STAT3 proteins was detected by western blotting (n = 3). Two-tailed Student’s t-test. (B)Fourteen days after OSM-induced osteogenic differentiation of tendon stem cells, expression of OSMR, GP130, p-JAK2, and p-STAT3 proteins was detected by western blotting (n = 3). Two-tailed Student’s t-test.Data are presented as means ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001

Article Snippet: The following primary mouse anti-rat monoclonal antibodies were purchased from Santa Cruz Biotechnology: OSMR (1:500, sc-376,511) and GP130 (1:500, sc-376,280).

Techniques: Expressing, Western Blot, Two Tailed Test, Standard Deviation

Fig. 5 OSM promotes osteogenic differentiation of tendon stem cells through the JAK2/STAT3 signalling pathway. OSM binds to the tendon stem cell receptor OSMR/GP130, activating and phosphorylating JAK2. Phosphorylated JAK2 activates STAT3 downstream, and phosphorylated STAT3 forms a dimer that translocates to the nucleus and binds to DNA to regulate gene transcription. AZD1480 and stattic inhibit activation and phosphorylation of JAK2 and STAT3, respectively

Journal: Journal of orthopaedic surgery and research

Article Title: Oncostatin M promotes osteogenic differentiation of tendon-derived stem cells through the JAK2/STAT3 signalling pathway.

doi: 10.1186/s13018-024-04915-5

Figure Lengend Snippet: Fig. 5 OSM promotes osteogenic differentiation of tendon stem cells through the JAK2/STAT3 signalling pathway. OSM binds to the tendon stem cell receptor OSMR/GP130, activating and phosphorylating JAK2. Phosphorylated JAK2 activates STAT3 downstream, and phosphorylated STAT3 forms a dimer that translocates to the nucleus and binds to DNA to regulate gene transcription. AZD1480 and stattic inhibit activation and phosphorylation of JAK2 and STAT3, respectively

Article Snippet: The following primary mouse anti-rat monoclonal antibodies were purchased from Santa Cruz Biotechnology: OSMR (1:500, sc-376,511) and GP130 (1:500, sc-376,280).

Techniques: Activation Assay, Phospho-proteomics